Route 326, or Highway 326, may refer to:
Canada Nova Scotia Route 326
China China National Highway 326
Costa Rica National Route 326
Japan Japan National Route 326
United States Interstate 326 (former) Arkansas Highway 326 Florida: Florida State Road 326 County Road 326 (Levy County, Florida) County Road 326 (Marion County, Florida) Georgia State Route 326 Kentucky Route 326 Louisiana Highway 326 Maryland Route 326 Minnesota State Highway 326 (former) Montana Secondary Highway 326 Montana Secondary Highway 326 (former) New York State Route 326 Ohio State Route 326 Pennsylvania Route 326 Puerto Rico Highway 326 Tennessee State Route 326 Texas: Texas State Highway 326 Texas State Highway Spur 326 Farm to Market Road 326 Virginia State Route 326