Sixteen Mile Creek or Sixteenmile Creek may refer to:
Canada Ontario Sixteen Mile Creek (Halton Region), which flows to the northwest shore of Lake Ontario Sixteen Mile Creek (Muskoka District), a tributary of the Boyne River Sixteen Mile Creek (Niagara Region), which flows to the southwest shore of Lake Ontario Sixteenmile Creek (Ontario), in Elgin County, which flows into Lake Erie
United States Sixteenmile Creek (Florida), a tributary of the St. Johns River Sixteen Mile Creek (Montana), a tributary of the Missouri River Sixteenmile Creek (Ohio), which flows into Lake Erie