James Humphrey may refer to:
James Humphrey (New York politician) (1811–1866), U.S. Representative from New York James Brown Humphrey (1859–1937), musician, bandleader, and music instructor in New Orleans, Louisiana James K. Humphrey, founder of the United Sabbath-Day Adventist Church, a small African American Christian denomination James M. Humphrey (1819–1899), also a U.S. Representative from New York James Humphrey (convict) (1832–1898), Australian convict and schoolteacher James Ellis Humphrey (1861–1897), American botanist and mycologist James H. Humphrey (1911–2008), educator and leader in the field of physical education
See also James Humphreys (disambiguation)