Many major cities and regional population centers in the United States lack any form of inter-city passenger rail service, which would typically be provided by Amtrak. Six metropolitan statistical areas of more than one million residents do not have inter-city train service, nor do the states of South Dakota or Wyoming. There are no international passenger trains between the United States and Mexico, nor to Canada outside of Ontario, Montreal, and Vancouver.

Cities lacking inter-city rail service For purposes of this list, a city is considered served if it is within of an Amtrak or other inter-city passenger rail station. However, some of these cities may be served directly by Amtrak Thruway services. Unless otherwise noted, the provider of the last passenger service is noted in each of the following cities was Amtrak. A partial list of the cities with a greater metropolitan population of over 100,000 that are not served by some form of inter-city rail service is as follows (in order by decreasing population):