Middlefield may refer to:
Canada Middlefield, Nova Scotia Middlefield Collegiate Institute, a high school in the area Middlefield Road, a street in Toronto a community in Markham, Ontario
England Middlefield, Stapleford, a mansion in Stapleford, Cambridgeshire
Scotland Middlefield, Aberdeen, a place in Aberdeen Middlefield, Falkirk, a U.K. location
United States Middlefield, Connecticut Middlefield, Massachusetts Middlefield, New York Middlefield, Ohio Middlefield station, a light rail station in Mountain View, California Middlefield Township, Buchanan County, Iowa Middlefield Township, Geauga County, Ohio