54th Regiment may refer to:

54th (City of London) Heavy Anti-Aircraft Regiment, Royal Artillery 54th (West Norfolk) Regiment of Foot 54th Foot, name of the 43rd (Monmouthshire) Regiment of Foot prior to 1751 54th Regiment of Foot, name of the 52nd (Oxfordshire) Regiment of Foot prior to 1756 54th Indiana Infantry Regiment 54th Infantry Regiment (France) 54th Infantry Regiment (Imperial Japanese Army) 54th Infantry Regiment (United States) 54th Massachusetts Infantry Regiment 54th Massachusetts Volunteer Regiment 54th New York Volunteer Infantry 54th Ohio Infantry 54th Pennsylvania Infantry Regiment 54th Regiment Kentucky Volunteer Mounted Infantry 54th United States Colored Infantry Regiment

See also 54th Infantry Regiment (disambiguation) 54th Division (disambiguation)