William Mayo may refer to:
William Benson Mayo (1866–1944), chief power engineer of the Ford Motor Company Two of the co-founders of the Mayo Clinic: William Worrall Mayo (1819–1911), British-American medical doctor and chemist, father of William James Mayo William James Mayo (1861–1939), American physician and surgeon William Starbuck Mayo (1812–1895), American doctor, traveler and writer William Mayo (civil engineer) (c. 1685–1744), who laid out the city of Richmond, Virginia William B. Mayo (politician), American doctor, businessman, and politician from Vermont
See also Bill Mayo (born 1963), American football player