Glutamate carboxypeptidase (, carboxypeptidase G, carboxypeptidase G1, carboxypeptidase G2, glutamyl carboxypeptidase, N-pteroyl-L-glutamate hydrolase) is an enzyme. This enzyme catalyses the following chemical reaction

Release of C-terminal glutamate residues from a wide range of N-acylating moieties, including peptidyl, aminoacyl, benzoyl, benzyloxycarbonyl, folyl and pteroyl groups

This zinc enzyme is produced by pseudomonads, Flavobacterium sp. and Acinetobacter sp.

See also Glutamate carboxypeptidase II

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