M54 or M-54 may be:
Roads: M54 motorway, a motorway in England also known as the Telford Motorway M-54 (Michigan highway), a state highway in Michigan M54 (Cape Town), a Metropolitan Route in Cape Town, South Africa M54 (Johannesburg), a Metropolitan Route in Johannesburg, South Africa
Vehicles: BMW M54, a 2000 automobile engine M54 (truck), a heavy truck used by the United States armed forces
Astronomy: Messier 54, a globular cluster in the constellation Sagittarius
Other: Samsung Galaxy M54 5G, a mid-range Android smartphone