Jacobs Well, Jacob's Well, or Jacobswell may refer to:
Structures Jacob's Well, or the Well of Sychar, a well mentioned in the New Testament and located in the West Bank Jacob's Well, Bristol, an early mediaeval structure in England that is thought to be a Jewish ritual bath Jacobs Well, York, a historic building in York, in England Jacob's Well, a well on the grounds of The Cross (Barre, Massachusetts)
Settlements Jacobs Well, Queensland, a suburb in City of Gold Coast, South-East Queensland, Australia Jacobs Well, Surrey, a village near Guildford, also called "Jacobswell"
Natural features Jacob's Well (Texas), a sinkhole and karstic spring located near Wimberley, Texas
Other Jacob's Well (sermon collection), 15th-century Middle English text