AOT may refer to:

Arts and entertainment Attack on Titan, a Japanese manga series by Hajime Isayama Attack on Titan (TV series), a Japanese anime series based on the manga

Science and technology Abstract object theory Aerosol OT, a common chemical reagent; see Dioctyl sodium sulfosuccinate Aerosol Optical Thickness, a measure for the concentration of aerosols determined by light absorption from remote sensing data Ahead-of-time compilation, an implementation approach for programming languages Assisted outpatient treatment, an implementation of mental-health law in the U.S.

Transport Aeronautical operations technician Airports of Thailand, an airport operator Aosta Airport (IATA code), Italy Autorité organisatrice de transports, a French legal structure for the provision of public transport A US Navy hull classification symbol: Transport oiler (AOT)

Other uses Academy of Technology, an engineering college in West Bengal, India A.O. Trikala, a football club in Trikala, Greece Archives Office of Tasmania, government archives office of Tasmania, Australia Army of Two (disambiguation)