U-27 may refer to one of the following German submarines:

, was the lead ship of the Type U 27 class of submarines; launched in 1913 and served in the First World War until sunk on 19 August 1915; the events surrounding U-27s sinking are known as the Baralong Incident During the First World War, Germany also had these submarines with similar names: , a Type UB II submarine launched in 1915 and sunk on 27 July 1917 , a Type UC II submarine launched in 1916 and surrendered on 3 February 1919 , a Type VIIA submarine that served in the Second World War until sunk on 20 September 1939 , a Type 206 submarine of the Bundesmarine that was launched in 1974 and scrapped in 1996