Pertwee is a surname. Notable people with the surname include:

Pertwee family of English actors/writers

Roland Pertwee (1885–1963), English writer and actor, father of Jon and Michael and uncle of Bill Michael Pertwee (1916–1991), English playwright Jon Pertwee (1919–1996), English actor best known as Doctor Who, father of Sean Bill Pertwee (1926–2013), English actor best known as the ARP Warden in Dad's Army Sean Pertwee (born 1964), English actor