Jackie O (Jacqueline Kennedy Onassis, 1929–1994) was the wife of the 35th President of the United States, John F. Kennedy.

Jackie O may also refer to:

Jackie O (opera), a 1997 opera by Michael Daugherty and Wayne Koestenbaum Jackie O (radio host) (Jacqueline Ellen Marie Henderson, born 1975), an Australian television presenter and radio personality

See also Jacki-O (born 1975), American rapper Jackie-O Motherfucker, an American experimental rock group