Route 394 or Highway 394 may refer to:
Canada Manitoba Provincial Road 394 Saskatchewan Highway 394
Japan Japan National Route 394
United States Interstate 394 Interstate 394 (Illinois) (possible future designation for Illinois Route 394) Arkansas Highway 394 Colorado State Highway 394 Illinois Route 394 Iowa Highway 394 (former) Maryland Route 394 (former) New York: New York State Route 394 County Route 394 (Erie County, New York) Pennsylvania Route 394 Puerto Rico Highway 394 Tennessee State Route 394 Virginia State Route 394