Route 240 or Highway 240 may refer to:
Australia Wimmera Highway
Canada Manitoba Provincial Road 240 Prince Edward Island Route 240 Saskatchewan Highway 240
Costa Rica National Route 240
Japan Japan National Route 240
United Kingdom road B240 road
United States Interstate 240 U.S. Route 240 (former) California State Route 240 (former) Florida State Road 240 (former) Georgia State Route 240 Hawaii Route 240 Indiana State Road 240 Kentucky Route 240 Massachusetts Route 240 Minnesota State Highway 240 (former) Missouri Route 240 Montana Secondary Highway 240 New Mexico State Road 240 New York State Route 240 Oregon Route 240 Pennsylvania Route 240 South Dakota Highway 240 Tennessee State Route 240 Texas State Highway 240 Texas State Highway Loop 240 Texas State Highway Spur 240 Utah State Route 240 Virginia State Route 240 Washington State Route 240 Wyoming Highway 240