E320 may refer to:
British Rail Class 374, or Eurostar e320, a European high-speed train Butylated hydroxyanisole (E number E320), a synthetic, waxy petrochemical FS Class E.320, a class of Italian third-rail electric locomotives, built in the 1910s ThinkPad E320, a 2011 Gen 2 Lenovo ThinkPad E series notebook computer E 320, a model in the Mercedes-Benz E-Class range of automobiles