Route 140 or Highway 140 may refer to:
Australia Hamilton Highway
Canada New Brunswick Route 140 Ontario Highway 140 Prince Edward Island Route 140
Costa Rica National Route 140
Germany Bundesautobahn 140
India National Highway 140 (India)
Japan Japan National Route 140 Fukuoka Prefectural Route 140 Nara Prefectural Route 140
Malaysia Malaysia Federal Route 140
United Kingdom road B140 road
United States Interstate 140 (two current highways) U.S. Route 140 (former) Alabama State Route 140 County Route 140 (Lee County, Alabama) Arkansas Highway 140 California State Route 140 Colorado State Highway 140 Connecticut Route 140 Florida State Road 140 (pre-1945) (former) Florida State Road 140A (pre-1945) (former) County Road 140 (Madison County, Florida) Georgia State Route 140 Illinois Route 140 Indiana State Road 140 Iowa Highway 140 K-140 (Kansas highway) Kentucky Route 140 Louisiana Highway 140 Maine State Route 140 Maryland Route 140 Massachusetts Route 140 M-140 (Michigan highway) Missouri Route 140 (former) Nevada State Route 140 New Hampshire Route 140 New Jersey Route 140 New Mexico State Road 140 New York State Route 140 County Route 140 (Broome County, New York) County Route 140 (Erie County, New York) County Route 140 (Fulton County, New York) County Route 140 (Montgomery County, New York) County Route 140 (Niagara County, New York) County Route 140 (Onondaga County, New York) County Route 140 (Rensselaer County, New York) County Route 140 (Wayne County, New York) North Carolina Highway 140 Ohio State Route 140 Oregon Route 140 Tennessee State Route 140 Texas State Highway 140 Texas State Highway Loop 140 (former) Texas State Highway Spur 140 Farm to Market Road 140 Utah State Route 140 Utah State Route 140 (pre-1969) (former) Vermont Route 140 Virginia State Route 140 Virginia State Route 140 (pre-1933) (former) Virginia State Route 140 (1933-1949) (former) Washington State Route 140 (former) West Virginia Route 140 Wisconsin Highway 140
Territories Puerto Rico Highway 140