The following highways are numbered 427:
Canada Manitoba Provincial Road 427 Ontario Highway 427
India National Highway 427 (India)
Japan Japan National Route 427
United States County Road 427 (Seminole County, Florida) Indiana State Road 427 County Road 427 (DeKalb County, Indiana) Nevada State Route 427 New York State Route 427 New York State Route 427 (former) Pennsylvania Route 427 Puerto Rico Highway 427 Texas State Highway Loop 427 (former)