List of EC numbers (EC 3)

This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology. All official information is tabulated at the website of the committee. The database is developed and maintained by Andrew McDonald.

EC 3.1: Acting on Ester Bonds

EC 3.1.1: Carboxylic Ester Hydrolases : carboxylesterase : arylesterase : triacylglycerol lipase : phospholipase A2 : lysophospholipase : acetylesterase : acetylcholinesterase : cholinesterase EC 3.1.1.9: deleted, a side reaction of cholinesterase : tropinesterase : pectinesterase EC 3.1.1.12: deleted, identical with carboxylesterase : sterol esterase : chlorophyllase : L-arabinonolactonase EC 3.1.1.16: deleted, mixture of (muconolactone Δ-isomerase) and (3-oxoadipate enol-lactonase) : gluconolactonase EC 3.1.1.18: deleted, now included with gluconolactonase : uronolactonase : tannase EC 3.1.1.21: deleted, now known to be catalysed by , carboxylesterase and , triacylglycerol lipase. : hydroxybutyrate-dimer hydrolase : acylglycerol lipase : 3-oxoadipate enol-lactonase : 1,4-lactonase : galactolipase : 4-pyridoxolactonase : acylcarnitine hydrolase : aminoacyl-tRNA hydrolase : D-arabinonolactonase : 6-phosphogluconolactonase : phospholipase A1 : 6-acetylglucose deacetylase : lipoprotein lipase : dihydrocoumarin hydrolase : limonin-D-ring-lactonase : steroid-lactonase : triacetate-lactonase : actinomycin lactonase : orsellinate-depside hydrolase : cephalosporin-C deacetylase : chlorogenate hydrolase : α-amino-acid esterase : 4-methyloxaloacetate esterase : carboxymethylenebutenolidase : deoxylimonate A-ring-lactonase : 1-alkyl-2-acetylglycerophosphocholine esterase : fusarinine-C ornithinesterase : sinapine esterase : wax-ester hydrolase : phorbol-diester hydrolase : phosphatidylinositol deacylase : sialate O-acetylesterase : acetoxybutynylbithiophene deacetylase : acetylsalicylate deacetylase : methylumbelliferyl-acetate deacetylase : 2-pyrone-4,6-dicarboxylate lactonase : N-acetylgalactosaminoglycan deacetylase : juvenile-hormone esterase : bis(2-ethylhexyl)phthalate esterase : protein-glutamate methylesterase EC 3.1.1.62: Now listed as , N-acetyldiaminopimelate deacetylase : 11-cis-retinyl-palmitate hydrolase : retinoid isomerohydrolase : L-rhamnono-1,4-lactonase : 5-(3,4-diacetoxybut-1-ynyl)-2,2′-bithiophene deacetylase : fatty-acyl-ethyl-ester synthase : xylono-1,4-lactonase EC 3.1.1.69: Now , N-acetylglucosaminylphosphatidylinositol deacetylase : cetraxate benzylesterase : acetylalkylglycerol acetylhydrolase : acetylxylan esterase : feruloyl esterase : cutinase : poly(3-hydroxybutyrate) depolymerase : poly(3-hydroxyoctanoate) depolymerase : acyloxyacyl hydrolase : polyneuridine-aldehyde esterase : hormone-sensitive lipase : acetylajmaline esterase : quorum-quenching N-acyl-homoserine lactonase : pheophorbidase : monoterpene ε-lactone hydrolase : cocaine esterase : pimelyl-(acyl-carrier protein) methyl ester esterase : rhamnogalacturonan acetylesterase : fumonisin B1 esterase : pyrethroid hydrolase : protein phosphatase methylesterase-1 : all-trans-retinyl ester 13-cis isomerohydrolase : 2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase : 4-sulfomuconolactone hydrolase : mycophenolic acid acyl-glucuronide esterase : versiconal hemiacetal acetate esterase : aclacinomycin methylesterase : D-aminoacyl-tRNA deacylase : methylated diphthine methylhydrolase : [Wnt protein] O-palmitoleoyl-L-serine hydrolase : 6-deoxy-6-sulfogluconolactonase : chlorophyllide a hydrolase : poly(ethylene terephthalate) hydrolase : mono(ethylene terephthalate) hydrolase : teichoic acid D-alanine hydrolase : 5-phospho-D-xylono-1,4-lactonase : 3-O-acetylpapaveroxine carboxylesterase : O-acetyl-ADP-ribose deacetylase : apo-salmochelin esterase : iron(III)-enterobactin esterase : iron(III)-salmochelin esterase : xylono-1,5-lactonase : phosphatidylserine sn-1 acylhydrolase : isoamyl acetate esterase : ethyl acetate hydrolase : methyl acetate hydrolase : D-apionolactonase : sn-1-specific diacylglycerol lipase : (4-O-methyl)-D-glucuronate—lignin esterase : phospholipid sn-1 acylhydrolase

EC 3.1.2: Thioester Hydrolases : acetyl-CoA hydrolase : palmitoyl-CoA hydrolase : succinyl-CoA hydrolase : 3-hydroxyisobutyryl-CoA hydrolase : hydroxymethylglutaryl-CoA hydrolase : hydroxyacylglutathione hydrolase : glutathione thiolesterase EC 3.1.2.8: Now included with hydroxyacylglutathione hydrolase EC 3.1.2.9: S-acetoacetylhydrolipoate hydrolase deleted : formyl-CoA hydrolase : acetoacetyl-CoA hydrolase : S-formylglutathione hydrolase : S-succinylglutathione hydrolase : oleoyl-[acyl-carrier-protein] hydrolase EC 3.1.2.15: covered by , ubiquitinyl hydrolase 1 : citrate lyase deacetylase : (S)-methylmalonyl-CoA hydrolase : ADP-dependent short-chain-acyl-CoA hydrolase : ADP-dependent medium-chain-acyl-CoA hydrolase : acyl-CoA hydrolase : dodecanoyl-(acyl-carrier-protein) hydrolase : palmitoyl[protein] hydrolase : 4-hydroxybenzoyl-CoA thioesterase EC 3.1.2.24: transferred entry now , 2′-hydroxybiphenyl-2-sulfinate desulfinase. : phenylacetyl-CoA hydrolase EC 3.1.2.26: Now , bile acid CoA transferase : choloyl-CoA hydrolase : 1,4-dihydroxy-2-naphthoyl-CoA hydrolase : fluoroacetyl-CoA thioesterase : (3S)-malyl-CoA thioesterase : dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase : 2-aminobenzoylacetyl-CoA thioesterase

EC 3.1.3: Phosphoric Monoester Hydrolases : alkaline phosphatase : acid phosphatase : phosphoserine phosphatase : phosphatidate phosphatase : 5′-nucleotidase : 3′-nucleotidase : 3′(2′),5′-bisphosphate nucleotidase : 3-phytase : glucose-6-phosphatase : glucose-1-phosphatase : fructose-bisphosphatase : trehalose-phosphatase EC 3.1.3.13: Recent studies have shown that this is a partial activity of , phosphoglycerate mutase (2,3-diphosphoglycerate-dependent) : methylphosphothioglycerate phosphatase : histidinol-phosphatase : protein serine/threonine phosphatase : (phosphorylase) phosphatase : phosphoglycolate phosphatase : glycerol-2-phosphatase : phosphoglycerate phosphatase : glycerol-1-phosphatase : mannitol-1-phosphatase : sugar-phosphatase : sucrose-phosphatase : inositol-phosphate phosphatase : 4-phytase : phosphatidylglycerophosphatase : ADP-phosphoglycerate phosphatase : N-acylneuraminate-9-phosphatase EC 3.1.3.30: The activity may be that of an acid phosphatase EC 3.1.3.31: The activity may be that of an acid phosphatase : polynucleotide 3′-phosphatase : polynucleotide 5′-phosphatase : deoxynucleotide 3′-phosphatase : thymidylate 5′-phosphatase : phosphoinositide 5-phosphatase : sedoheptulose-bisphosphatase : 3-phosphoglycerate phosphatase : streptomycin-6-phosphatase : guanidinodeoxy-scyllo-inositol-4-phosphatase : 4-nitrophenylphosphatase : [glycogen-synthase-D] phosphatase : [pyruvate dehydrogenase (acetyl-transferring)]-phosphatase : [acetyl-CoA carboxylase]-phosphatase : 3-deoxy-manno-octulosonate-8-phosphatase : fructose-2,6-bisphosphate 2-phosphatase : [hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase : protein-tyrosine-phosphatase : [pyruvate kinase]-phosphatase : sorbitol-6-phosphatase : dolichyl-phosphatase : [3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]-phosphatase : [myosin-light-chain] phosphatase : fructose-2,6-bisphosphate 6-phosphatase : caldesmon-phosphatase : inositol-polyphosphate 5-phosphatase : inositol-1,4-bisphosphate 1-phosphatase : sugar-terminal-phosphatase : alkylacetylglycerophosphatase : phosphoenolpyruvate phosphatase EC 3.1.3.61: deleted, as its existence has not been established : multiple inositol-polyphosphate phosphatase : 2-carboxy-D-arabinitol-1-phosphatase : phosphatidylinositol-3-phosphatase EC 3.1.3.65: Now included with , phosphatidylinositol-3-phosphatase : phosphatidylinositol-3,4-bisphosphate 4-phosphatase : phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase : 2-deoxyglucose-6-phosphatase : glucosylglycerol 3-phosphatase : mannosyl-3-phosphoglycerate phosphatase : 2-phosphosulfolactate phosphatase : 5-phytase : adenosylcobalamin/α-ribazole phosphatase : pyridoxal phosphatase : phosphoethanolamine/phosphocholine phosphatase : lipid-phosphate phosphatase : acireductone synthase : phosphatidylinositol-4,5-bisphosphate 4-phosphatase : mannosylfructose-phosphate phosphatase : 2,3-bisphosphoglycerate 3-phosphatase EC 3.1.3.81: Transferred entry, now , diacylglycerol diphosphate phosphatase : D-glycero-β-D-manno-heptose 1,7-bisphosphate 7-phosphatase : D-glycero-α-D-manno-heptose 1,7-bisphosphate 7-phosphatase : ADP-ribose 1″-phosphate phosphatase : glucosyl-3-phosphoglycerate phosphatase : phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase : 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase : 5′′-phosphoribostamycin phosphatase

EC 3.1.4: Phosphoric Diester Hydrolases : phosphodiesterase I : glycerophosphocholine phosphodiesterase : lecithinase C : phospholipase D EC 3.1.4.5: Now , deoxyribonuclease I EC 3.1.4.6: Now , deoxyribonuclease II EC 3.1.4.7: Now , micrococcal nuclease EC 3.1.4.8: Now , ribonuclease T1 EC 3.1.4.9: Now , Serratia marcescens nuclease EC 3.1.4.10: Now , phosphatidylinositol diacylglycerol-lyase : phosphoinositide phospholipase C : sphingomyelin phosphodiesterase : serine-ethanolaminephosphate phosphodiesterase : [acyl-carrier-protein] phosphodiesterase : transferred to EC 2.7.7.89, adenylyl-[glutamateammonia ligase] phosphorylase : 2′,3′-cyclic-nucleotide 2′-phosphodiesterase : 3′,5′-cyclic-nucleotide phosphodiesterase EC 3.1.4.18: Now , spleen exonuclease EC 3.1.4.19: Now , oligonucleotidase EC 3.1.4.20: Now , exoribonuclease II EC 3.1.4.21: Now , Aspergillus nuclease S1 EC 3.1.4.22: Now , pancreatic ribonuclease EC 3.1.4.23: Now , ribonuclease T2 EC 3.1.4.24: deleted EC 3.1.4.25: Now , exodeoxyribonuclease I EC 3.1.4.26: deleted EC 3.1.4.27: Now , exodeoxyribonuclease III EC 3.1.4.28: Now , exodeoxyribonuclease (lambda-induced) EC 3.1.4.29: deleted EC 3.1.4.30: Now , deoxyribonuclease IV (phage-T4-induced) EC 3.1.4.31: Now EC 3.1.4.32: deleted EC 3.1.4.33: deleted EC 3.1.4.34: deleted : 3′,5′-cyclic-GMP phosphodiesterase EC 3.1.4.36: Now with : 2′,3′-cyclic-nucleotide 3'-phosphodiesterase : glycerophosphocholine cholinephosphodiesterase : alkylglycerophosphoethanolamine phosphodiesterase : CMP-N-acylneuraminate phosphodiesterase : sphingomyelin phosphodiesterase D : glycerol-1,2-cyclic-phosphate 2-phosphodiesterase : glycerophosphoinositol inositolphosphodiesterase : glycerophosphoinositol glycerophosphodiesterase : N-acetylglucosamine-1-phosphodiester α-N-acetylglucosaminidase : glycerophosphodiester phosphodiesterase EC 3.1.4.47: Now , glycosylphosphatidylinositol diacylglycerol-lyase : dolichylphosphate-glucose phosphodiesterase : dolichylphosphate-mannose phosphodiesterase : glycosylphosphatidylinositol phospholipase D : glucose-1-phospho-D-mannosylglycoprotein phosphodiesterase : cyclic-guanylate-specific phosphodiesterase : 3′,5′-cyclic-AMP phosphodiesterase : N-acetylphosphatidylethanolamine-hydrolysing phospholipase D : phosphoribosyl 1,2-cyclic phosphate phosphodiesterase : 7,8-dihydroneopterin 2′,3′-cyclic phosphate phosphodiesterase : phosphoribosyl 1,2-cyclic phosphate 1,2-diphosphodiesterase : RNA 2′,3′-cyclic 3′-phosphodiesterase : cyclic-di-AMP phosphodiesterase : pApA phosphodiesterase : cyclic 2,3-diphosphoglycerate hydrolase

EC 3.1.5: Triphosphoric Monoester Hydrolases : dGTPase

EC 3.1.6: Sulfuric Ester Hydrolases : arylsulfatase (type I) : steryl-sulfatase : glycosulfatase : N-acetylgalactosamine-6-sulfatase EC 3.1.6.5: deleted : choline-sulfatase : cellulose-polysulfatase