Die Liste der Eisenbahngesellschaften in den Vereinigten Staaten bietet eine Übersicht über Eisenbahngesellschaften, die Güter-, Personen- und Touristenverkehre anbieten.
2017 existierten in den Vereinigten Staaten 614 öffentliche Eisenbahngesellschaften, die Gütertransport betrieben. Entsprechend der Einteilung der Association of American Railroads (AAR) waren es zum 31. Dezember 2009 7 Class-1-Gesellschaften, 23 Regional Railroads, 339 Local Railroads, 194 Rangiergesellschaften und zwei kanadische Gesellschaften (CN und CP).
Class 1 – Eisenbahngesellschaften Eisenbahnen mit einem Umsatz von mehr als 401,4 Millionen Dollar BNSF Railway CSX Transportation Kansas City Southern Railway, Tochtergesellschaft der Canadian Pacific Kansas City Norfolk Southern Railway Union Pacific Railroad Soo Line Railroad, Tochtergesellschaft der Canadian Pacific Kansas City Grand Trunk Corporation, Tochtergesellschaft der Canadian National Railway
Regional Railroads (Class 2) Eine Eisenbahngesellschaft, die eine Strecke von mehr als 350 Meilen betreibt und/oder mehr als 40 Mio. $ Umsatz hat. Alabama and Gulf Coast Railway Alaska Railroad Buffalo and Pittsburgh Railroad Dakota, Missouri Valley and Western Railroad Florida East Coast Railway Indiana Rail Road Iowa Interstate Railroad Kansas and Oklahoma Railroad Kyle Railroad Montana Rail Link Nebraska Kansas Colorado Railway New England Central Railroad New York, Susquehanna and Western Railway Paducah and Louisville Railway Pan Am Railways Portland and Western Railroad Providence and Worcester Railroad Red River Valley and Western Railroad Wheeling and Lake Erie Railway Wisconsin and Southern Railroad
Local Railroads (Class 3) keine „Class 1“- oder „Class 2“-Eisenbahn, aber mit fahrplanmäßigen Betrieb
Aberdeen and Rockfish Railroad Aberdeen, Carolina and Western Railway Acadiana Railway Alabama Southern Railway Alabama Warrior Railway Alabama and Tennessee River Railway Albany and Eastern Railroad Alexander Railroad Aliquippa and Ohio River Railroad AN Railway Angelina and Neches River Railroad Ann Arbor Railroad Apache Railway Appalachian and Ohio Railroad Arcade and Attica Railroad Arizona and California Railroad Arizona Central Railroad (Clarkdale Arizona Central Railroad) Arkansas and Missouri Railroad Arkansas and Oklahoma Railroad Arkansas, Louisiana and Mississippi Railroad Arkansas Midland Railroad Arkansas Southern Railroad Ashland Railway Athens Line AT&L Railroad Atlantic and Western Railway B&H Rail Batten Kill Railroad Bauxite and Northern Railway Bay Coast Railroad Bay Colony Railroad Bay Line Railroad Belvidere and Delaware River Railway Blacklands Railroad Blackwell and Northern Gateway Railway Bloomer Line Boise Valley Railroad Border Pacific Railroad Buckingham Branch Railroad Butte, Anaconda and Pacific Railway Caddo Valley Railroad Caldwell County Railroad Caney Fork and Western Railroad Carolina Coastal Railway Carolina Southern Railroad Carrizo Gorge Railway Cascade and Columbia River Railroad Cedar Rapids and Iowa City Railway Central Maine and Quebec Railway Central Montana Rail Central New England Railroad Central New York Railroad Central Oregon and Pacific Railroad Central Railroad Company of Indiana Central Railroad Company of Indianapolis Central Washington Railroad Chattahoochee Bay Railroad Chattahoochee Industrial Railroad Chattooga and Chickamauga Railway Chesapeake and Albemarle Railroad Chesapeake and Indiana Railroad Chicago, Ft. Wayne and Eastern Railroad Chicago, SouthShore and South Bend Railroad City of Prineville Railway Claremont Concord Railroad Clarendon and Pittsford Railroad Cleveland Commercial Railroad Columbia Basin Railroad Company Columbia and Cowlitz Railway Columbus and Greenville Railway Columbus and Ohio River Rail Road Commonwealth Railway Conecuh Valley Railroad Connecticut Southern Railroad Copper Basin Railway D&I Railroad Dakota Northern Railroad Dardanelle and Russellville Railroad De Queen and Eastern Railroad Delaware-Lackawanna Railroad Delta Southern Railroad Denver and Rio Grande Railroad Depew, Lancaster and Western Railroad Dover & Rockaway River Railroad Dubois County Railroad East Cooper and Berkeley Railroad East Tennessee Railway Eastern Alabama Railway Eastern Idaho Railroad Eastern Maine Railway Eastern Washington Gateway Railroad Effingham Railroad El Dorado and Wesson Railway Elkhart and Western Railroad Escanaba and Lake Superior Railroad Evansville Western Railway Everett Railroad Falls Road Railroad Finger Lakes Railway First Coast Railroad Florida Central Railroad (1986) Florida Gulf & Atlantic Railroad Florida Midland Railroad Florida Northern Railroad Fulton County LLC Fulton County Railway Georgetown Railroad Georgia Central Railway Georgia Midland Railroad Georgia Northeastern Railroad Georgia Southwestern Railroad Georgia Woodlands Railroad Georgia and Florida Railway Gettysburg and Northern Railroad Golden Isles Terminal Railroad Golden Triangle Railroad Grainbelt Grand Elk Railroad Grand Rapids Eastern Railroad Great Lakes Central Railroad Great Northwest Railroad Great Smoky Mountains Railroad Great Walton Railroad Great Western Railway of Colorado Green Mountain Railroad Greenville and Western Railway Grenada Railway Gulf, Colorado and San Saba Railway Hampton and Branchville Railroad Hartwell Railroad Heart of Georgia Railroad Hollidaysburg and Roaring Spring Railroad Hollis and Eastern Railroad Hoosier Southern Railroad Housatonic Railroad Huntsville and Madison County Railroad Authority Huron and Eastern Railway Idaho Northern and Pacific Railroad Illinois Railway Illinois and Midland Railroad Illinois Western Railroad Indiana and Ohio Railway Indiana Eastern Railroad Indiana Northeastern Railroad Indiana Southern Railroad Iowa Northern Railway Iowa River Railroad Iowa Traction Railroad Ithaca Central Railroad Juniata Valley Railroad Kankakee, Beaverville and Southern Railroad Kaw River Railroad Kentucky West Tennessee Railway Keokuk Junction Railway Kettle Falls International Railway Kiamichi Railroad Company Kosciusko and Southwestern Railway Lake State Railway Lake Superior and Ishpeming Railroad Lancaster and Chester Railway Landisville Railroad Laurinsburg and Southern Railroad Lehigh Railway Little Rock and Western Railway Livonia, Avon and Lakeville Railroad Louisiana Southern Railroad Louisiana and Delta Railroad Louisiana and North West Railroad Louisville and Indiana Railroad Lowville and Beaver River Railroad Luxapalila Valley Railroad Lycoming Valley Railroad Madison Railroad Maine Eastern Railroad Marquette Rail Maryland Midland Railway Maryland and Delaware Railroad Massachusetts Central Railroad McCloud Railway Meridian and Bigbee Railroad Meridian Southern Railway Mid-Michigan Railroad Middletown and New Jersey Railway Middletown and Hummelstown Railroad Milford-Bennington Railroad Minnesota Northern Railroad Minnesota Prairie Line Minnesota Southern Railway Minnesota, Dakota and Western Railway Mission Mountain Railroad Mississippi Export Railroad Mississippi Southern Railroad Mississippian Railway Cooperative Missouri and Northern Arkansas Railroad Mohawk, Adirondack and Northern Railroad Morristown and Erie Railway Mount Hood Railroad Nash County Railroad Nashville and Eastern Railroad Natchez Railroad Naugatuck Railroad NC Railroad NEBKOTA Railway Nebraska Central Railroad Nebraska Northeastern Railway New England Southern Railroad New Hampshire Central Railroad New Hampshire Northcoast New Hope Railroad New Jersey Rail Carrier New York and Atlantic Railway New York and Greenwood Lake Railway New York and Lake Erie Railroad New York and Ogdensburg Railway Nittany and Bald Eagle Railroad North Shore Railroad Northern Plains Railroad Ohio Central Railroad Ohio Southern Railroad Ohio Valley Railroad Ohio and Pennsylvania Railroad Oil Creek and Titusville Lines Old Augusta Railroad Ontario Central Railroad Ontario Midland Railroad Otter Tail Valley Railroad Ouachita Railroad Ozark Valley Railroad Pacific Sun Railroad Palouse River and Coulee City Railroad Pan Am Southern Panhandle Northern Railroad Pecos Valley Southern Railway Pee Dee River Railway Pend Oreille Valley Railroad Pennsylvania and Southern Railway Pennsylvania Northeastern Railroad Pickens Railway Pickens Railway Co. – Honea Path Division Pioneer Valley Railroad Point Comfort and Northern Railway Prescott and Northwestern Railroad Progressive Rail Puget Sound and Pacific Railroad R. J. Corman Railroad/Bardstown Line R. J. Corman Railroad/Central Kentucky Lines R. J. Corman Railroad/Cleveland Line R. J. Corman Railroad/Memphis Line R. J. Corman Railroad/Pennsylvania Line R. J. Corman Railroad/TN Terminal LLC R. J. Corman Railroad/West Virginia Line R. J. Corman Railroad/Western Ohio Line Reading Blue Mountain and Northern Railroad Redmont Railway Riceboro Southern Railway Rochester and Southern Railroad Rock and Rail Rockdale, Sandow and Southern Railroad Sabine River and Northern Railroad Sacramento Valley Railroad Saginaw Bay Southern Railway Salt Lake, Garfield and Western Railway San Diego and Imperial Valley Railroad San Joaquin Valley Railroad San Luis Central Railroad San Luis and Rio Grande Railroad Sand Springs Railway Sandersville Railroad Santa Fe Southern Railway Santa Maria Valley Railroad Seminole Gulf Railway Sequatchie Valley Railroad Shamokin Valley Railroad Shenandoah Valley Railroad Sierra Northern Railway Sisseton Milbank Railroad SMS Rail Service SMS Rail Lines of New York South Branch Valley Railroad South Central Florida Express South Central Tennessee Railroad South Kansas and Oklahoma Railroad Southern Indiana Railway Southern Railroad Co. of NJ Squaw Creek Southern Railroad St. Croix Valley Railroad St. Lawrence and Atlantic Railroad St. Marys Railroad St. Paul & Pacific Railroad St. Paul & Pacific Northwest Railroad Stillwater Central Railroad Stockton Terminal and Eastern Railroad Stourbridge Railroad Sunflour Railroad Tacoma Rail Mountain Division Tennessee Southern Railroad Tennken Railroad Texas-New Mexico Railroad Texas North Western Railway Texas Northeastern Railroad Texas-Pacífico Transportation Texas Rock Crusher Railway Texas and Northern Railway Thermal Belt Railway Three Notch Railroad Timber Rock Railroad Toledo, Peoria and Western Railway Tomahawk Railway Towner Railway Trona Railway Tulsa-Sapulpa Union Railway Twin Cities and Western Railroad Tyburn Railroad Union County Industrial Railroad Utah Railway Utah Southern Railway V and S Railway Valdosta Railway Vandalia Railroad Ventura County Railroad Vermont Railway Vicksburg Southern Railroad Walking Horse and Eastern Railroad Wallowa Union Railroad Authority Warren and Saline River Railroad Washington and Idaho Railway Washington County Railroad Wellsboro and Corning Railroad West Isle Line West Tennessee Railroad West Texas and Lubbock Railroad West Virginia Central Railroad Western New York and Pennsylvania Railroad Western Rail Road Williamette Valley Railway Williamette and Pacific Railroad Winchester and Western Railroad Wiregrass Central Railroad Wisconsin Northern Wyoming and Colorado Railroad Yadkin Valley Railroad YCR Corporation Yellowstone Valley Railroad York Railway Youngstown and Southeastern Railroad Yreka Western Railroad
Rangiergesellschaften Eisenbahnen, die keine Punkt-zu-Punkt-Transporte (Linienverkehr) durchführen, sondern die Verbindung zwischen Endabnehmern und den anderen Eisenbahngesellschaften herstellen. Adrian and Blissfield Rail Road Akron Barberton Cluster Railway Alabama Railroad Alabama and Florida Railway Alamo Gulf Coast Railroad Albany Port Railroad